Parasites and health [Last Modified: ]
Intestinal parasites: Comparative morphology

Table 2
Characteristics of Intestinal Flagellates, Ciliate, and Coccidia Visible in Different Types of Fecal Preparations

 
 

UNSTAINED

TEMPORARY STAINS

PERMANENT STAINS

Characteristic

Saline

Formalin

Iodine
(Cysts)

Neutral Red 1
(Trophs)

 
Flagellates

Trophozoites

 
Motility

+

­

 

+

­

Shape

+

+

 

+

+
(may be distorted)

Nucleus

­

+

 

+

+

Flagella

±

­

 

+

±

Other features2

+

+

 

+

+

Cysts

 
Shape

+

+

+

 

+

Nuclei

­

+

+

 

+

Fibrils

±

+

+

 

+

Ciliate (Balantidium coli)

Trophozoites

 
Motility

+

­

 

+

­

Macronucleus

+

+

 

+

+

Cilia

+

+

 

+

+

Cysts

 
Macronucleus

+

+

±

 

+

Coccidia

Oocysts/Sporocysts

+

+

+

 

±3

1Neutral red dye in methocel solutions.
2The undulating membrane of Trichomonas and the spiral groove of Chilomastix may not be visible in all cases.
3Cryptosporidium oocysts can be demonstrated in acid-fast stains.

 

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